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In situ hybridization in hematopathology

Strickler, J.G.; Copenhaver, C.M.

American Journal of Clinical Pathology 93(4 Suppl 1): S44-S48

1990

ISSN/ISBN: 0002-9173
PMID: 1690505
Document Number: 365742
In situ hybridization (ISH) is one of the molecular techniques that has applications in diagnostic hematopathology. This procedure allows the detection of DNA or RNA in intact cells from various preparations, including cytology specimens and routinely fixed paraffin-embedded tissues. ISH is therefore analogous to detecting proteins (antigens) in intact cells with immunohistochemistry. The purpose of this article is to review the basic concepts and principles of ISH and to briefly discuss the important technical details of this procedure. Examples of potential applications of ISH in hematopathology are then discussed, including detection of Epstein-Barr virus, Y chromosome, and oncogene activation.

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Choi, Y.J. 1990: In situ hybridization using a biotinylated DNA probe on formalin-fixed liver biopsies with hepatitis B virus infections: in situ hybridization superior to immunochemistry Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 3(3): 343-347
Kawai, H.; Kaneko, S.; Kobayashi, K. 1995: Detection of hepatitis C virus (HCV) infected cell using in situ hybridization and PCR in situ hybridization Nihon Rinsho. Japanese Journal of Clinical Medicine 53 Suppl (Part 1): 307-311
Mahdy, E.; Yoshihiro, S.; Zech, L.; Wester, K.; Pan, Y.; Busch, C.; Döhner, H.; Kallioniemi, O.; Bergerheim, U.; Malmström, P.U. 1999: Comparison of comparative genomic hybridization, fluorescence in situ hybridization and flow cytometry in urinary bladder cancer Anticancer Research 19(1a): 7-12
Komminoth, P.; Adams, V.; Long, A.A.; Roth, J.; Saremaslani, P.; Flury, R.; Schmid, M.; Heitz, P.U. 1994: Comparison of histology, immunohistochemistry, RT-PCR, in situ hybridization, and in situ RT-PCR for demonstration of hepatitis C virus in paraffin-embedded liver biopsies Verhandlungen der Deutschen Gesellschaft für Pathologie 78: 220-225
Shapshak, P.; Sun, N.C.; Resnick, L.; Hsu, M.Y.; Tourtellotte, W.W.; Schmid, P.; Conrad, A.; Fiala, M.; Imagawa, D.T. 1990: The detection of HIV by in situ hybridization Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 3(2): 146-153
Wakamiya, N.; Horiguchi, J.; Kufe, D. 1987: Detection of c-fms and CSF-1 RNA by in situ hybridization Leukemia 1(6): 518-520
Nürnberg, W.; Czarnetzki, B.M.; Schadendorf, D. 1995: DIG-labeled RNA in situ hybridization without coverslipping Biotechniques 18(3): 406-408
Nakane, P.K.; Moriuchi, T.; Koji, T. 1986: Enzyme-immuno-histo in situ hybridization Gan to Kagaku Ryoho. Cancer and ChemoTherapy 13(3 Part 2): 740-746
McLaughlin, S.K.; Margolskee, R.F. 1993: 33P is preferable to 35S for labeling probes used in in situ hybridization Biotechniques 15(3): 506-511
de Boer, J.M.; Yan, Y.; Smant, G.; Davis, E.L.; Baum, T.J. 1998: In-situ Hybridization to Messenger RNA in Heterodera glycines Journal of Nematology 30(3): 309-312
Mullink, H. 1991: In situ hybridization. Principles and applications in pathology Zeitschrift für Erkrankungen der Atmungsorgane 176(2-3): 172-173
Jikai, J.; Ryo, R.; Yasunaga, M.; Sugano, W.; Yamaguchi, N.; Hirano, T. 1992: Detection of mRNA in megakaryocytes using in situ hybridization Rinsho Byori. Japanese Journal of Clinical Pathology 40(8): 841-847
Michalová, K. 1995: Fluorescent in situ hybridization in clinical cytogenetics Casopis Lekaru Ceskych 134(3): 73-76
Sarasophona, S.; Promsawat, K.; Rerkamnuaychok, B. 2000: Identification of sex chromosome by fluorescence in situ hybridization Journal of the Medical Association of Thailand 83(Suppl 1): S89-S94
Zakut, H.; Zamir, R.; Soreq, H. 1989: In-situ hybridization on chorionic villi chromosomes Harefuah 116(2): 81-85
Hou, J.W.; Tsai, W.Y.; Wang, T.R. 1999: Detection of KAL-1 gene deletion with fluorescence in situ hybridization Journal of the Formosan Medical Association 98(6): 448-451
Multani, A.S.; Hopwood, V.L.; Pathak, S. 1996: A modified fluorescence in situ hybridization (FISH) technique Anticancer Research 16(6b): 3435-3437
Gole, L.A.; Bongso, A. 1997: Fluorescent in-situ hybridization--some of its applications in clinical cytogenetics Singapore Medical Journal 38(11): 497-503
Domracheva, E.V.; Kobzev, I.N. 1997: The use of fluorescent in situ hybridization (FISH) in chronic myeloleukemia Terapevticheskii Arkhiv 69(7): 71-74
Shin, M.; Koji, T. 1998: Detection of receptor mRNAs using nonradioactive in situ hybridization Nihon Rinsho. Japanese Journal of Clinical Medicine 56(7): 1667-1673