Induction of quiescence by differentiating agents
Gorisse, M.C.; Carpentier, Y.; Desoize, B.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 184(5-6): 363-369
1990
ISSN/ISBN: 0037-9026 PMID: 2151808 Document Number: 365383
The growth fraction of cancer cells, estimated by the monoclonal antibody Ki-67 labelling, and DNA content were determined simultaneously en K562 human leukemic cells by flow cytometry. Adriamycin, aclacinomycin A and fagaronine induced differentiation, as assessed by benzidine staining and glycophorin A expression. These drugs decreases the fraction of Ki-67 positive cells, Ki-67 negative cells displayed a G1, but also a G2 and a S DNA content in different proportions, indicating that induction of quiescent cells by differentiating agents is not a uniform process and is worthy of interest.