Sequence of a cDNA encoding human galactose-1-phosphate uridyl transferase

Flach, J.E.; Reichardt, J.K.; Elsas, L.J.

Molecular Biology and Medicine 7(4): 365-369

1990


ISSN/ISBN: 0735-1313
PMID: 2233247
Document Number: 364624
We report a revised sequence of a cDNA that encodes a human galactose-1-phosphate uridyl transferase. The cDNA is 1295 bases in length and encodes a 43,000 Mr protein. The sequence was derived from a cDNA clone isolated from a transformed human lymphoblast cell line and amplified in a polymerase chain reaction. The revised sequence reveals a higher degree of amino acid conservation between the human enzyme and the homologous enzymes from Escherichia coli and yeast than was previously thought to exist.

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