Effect of tuftsin and hydroxymethacil on tyrosine hydrolase of macrophages in chronic stress
Mineeva, M.F.; Bulatova, N.R.; Val'dman, A.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 109(4): 327-330
1990
ISSN/ISBN: 0365-9615 PMID: 1974817 Document Number: 361446
It has been established that rat peritoneal macrophages possess tyrosine hydroxylase activity which has been characterized by KM4, 2 microM, Vmax 30 nmole/min per mg of protein. After 15 days of electronociseptive stimulation almost all tyrosine hydroxylase activity has been established in the form which has tyrosine KM47.0 M and Vmax 18.6 nmole/min. per mg of protein. Immunostimulator hydroxymethacil++, at its intraperitoneal injections to stressed rats during 7 days induced the appearance of low-affinity form of tyrosine hydroxylase with KM270 microM and Vmax 27.8 nmole/min.per mg of protein. The same low affinity form of the enzyme has been established after injections of tuftsin which possesses immunostimulating properties.