Kinetic modeling of intracellular pH and comparison with 31P NMR experimental values in dialysed uremic patients
Monti, J.P.; Sarrazin, M.; Gallice, P.; Crevat, A.; Baz, M.; Murisasco, A.; Elsen, R.
International Journal of Artificial Organs 13(12): 799-802
1990
ISSN/ISBN: 0391-3988 PMID: 2289832 Document Number: 360943
Changes in intra-erythrocytic pH values over time, during and after bicarbonate hemodialysis, were studied with 31P Nuclear Magnetic Resonance. Simultaneously, pH values of whole blood were obtained by a gazometric method. A two-compartment model appeared to be the simplest kinetic model to explain the shifts in proton concentrations in extra- and intra-cellular media. Non-linear regression was used to determine exchange constant values. There was a very good correlation between the experimental and calculated proton concentrations. This model can describe all patients but individual experimental constants must be determined. Under these conditions a single blood pH determination before dialysis will permit determination of the initial intra-erythrocytic pH and monitoring of intra-erythrocytic pH during hemodialysis.