Quantitative measurement of guinea pig complement with liposomes
Knudson, K.C.; Bing, D.H.; Kater, L.
Journal of Immunology 106(1): 258-265
1971
ISSN/ISBN: 0022-1767 PMID: 5543717 Document Number: 36062
Liposomes were investigated as an alternative reagent to sheep erythrocytes for quantitative titration of whole complement activity. The liposomes were prepared with various concentrations of phos-pholipid and were sonicated for more effective dispersion. It was found that reproducible complement titrations could be performed with sonicated liposomes prepared with 1 or 2 micro mol of phospholipid per ml of marker solution (0.3 M galactose).
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