Lysosomotropic agents inhibit the penetration of arenaviruses into a culture of BHK-21 and Vero cells
Glushakova, S.E.; Iakuba, A.I.; Vasiuchkov, A.D.; Mar'iankova, R.F.; Kukareko, T.M.; Stel'makh, T.A.; Kurash, T.P.; Lukashevich, I.S.
Voprosy Virusologii 35(2): 146-150
1990
ISSN/ISBN: 0507-4088 PMID: 2389568 Document Number: 355755
Lysosomotropic agents (NH4Cl, amantadine, chloroquine, monensin) which prevent acidification of intracellular vacuoles, when introduced into the culture medium before or during inoculation of cells (BHK-21, Vero) with arenaviruses inhibit reproduction of these viruses completely or significantly. Mozambique virus proved to be 10 times more sensitive to the effect of lysosomotropic agents than Pichinde and Lassa viruses. Thus, arenaviruses have a pH-dependent stage at the beginning of the reproduction cycle which is indirectly indicative of their penetration into cells by receptor endocytosis.