Cryosectioning of hair follicles. An improved method using liquid nitrogen conduction freezing
Van Baar, H.M.; Schalkwijk, J.; Perret, C.M.
Acta Dermato-Venereologica 70(4): 335-338
1990
ISSN/ISBN: 0001-5555 PMID: 1977260 Document Number: 353086
Horizontal sectioning of scalp biopsies is especially useful in hair diseases characterized by a reduction of follicles in size or number. Horizontal sectioning using standard cryo-microtomes is hampered by the differences in cutting properties between hair follicles and fatty tissue, resulting in loss of topography. We report a simple, inexpensive method to temporarily cool the specimen to an appropriate temperature by means of a conduction system using liquid nitrogen. Immunohistological methods such as immunofluorescence and immunoperoxidase techniques can be applied without restrictions.