Role of a collagenase in the latent proteolytic activity of fibronectin
Imhoff, J.M.; Blondeau, X.; Planchenault, T.; Emod, I.; Keil-Dlouha, V.
Pathologie-Biologie 38(10): 999-1004
1990
ISSN/ISBN: 0369-8114 PMID: 1963217 Document Number: 351537
Fibronectin fragments generated by Achromobacter iophagus collagenase exhibit a gelatinolytic activity. This activity is inhibited by phenyl-methyl-sulfonyl fluoride and pepstatin A. After separation of this collagenase digest of fibronectin on heparin Ultrogel, a laminase activity was also evidenced using laminin and the synthetic peptide Gly-Pro-Ala-Gly-Pro-Arg as substrates. Different results were obtained with a cathepsin D digest of fibronectin that exhibited gelatinolytic and laminolytic activities only after incubation with Ca++. This suggests that the proteinases produced by hydrolysis of fibronectin enhance the effect of collagenase on extracellular matrix proteins.