Efficient Procedure for Extracting Tylenchulus semipenetrans from Citrus Roots
Greco, N.; D'Addabbo, T.
Journal of Nematology 22(4): 590-593
1990
ISSN/ISBN: 0022-300X PMID: 19287763 Document Number: 351348
Investigations were undertaken to determine the suitability of sucrose and magnesium sulfate solutions and a silica colloidal suspension with centrifugation for extracting T. semipenetrans from Citrus aurantium roots. The efficiency of incubation, sodium hypochlorite, centrifugation, and maceration methods was also compared. Numbers of females recovered by centrifugation with colloidal silica were greater than those from sucrose or magnesium sulfate. Incubation, sodium hypochlorite, and centrifugation methods were satisfactory for extracting eggs, 2nd-stage juveniles, and males, whereas the maceration-sieving method was less efficient. Combining the sodium hypochlorite method with a 15-second maceration followed by centrifugation in colloidal silica reduced the recovery of T. semipenetrans females from citrus roots.