Development of ELISPOT assay for thyroid autoantibody-producing cells
Kaneda, T.; Iwatani, Y.; Amino, N.; Tamaki, H.; Hidaka, Y.; Suehisa, E.; Hosotsubo, H.; Hayashi, J.; Miyai, K.
Rinsho Byori. Japanese Journal of Clinical Pathology 38(8): 883-887
1990
ISSN/ISBN: 0047-1860 PMID: 2232248 Document Number: 351082
A new assay system for detection of thyroid-autoantibody-producing cells was developed. This assay is based on the ELISPOT method with antigen-coated nitrocellulose membranes in 96-well microfilter plates. This was more sensitive than conventional methods such as a radioimmunoassay and a passive agglutination method for detection of thyroid-autoantibody production. The coefficients of inter- and intra-assay variations for antibody-producing cells were less than 6.5%. Thus, this assay system can be used to analyse the thyroid-specific immunological abnormalities as a routine test.