Shampoo residue profiles in human head hair
Andrasko, J.; Stocklassa, B.
Journal of Forensic Sciences 35(3): 569-579
1990
ISSN/ISBN: 0022-1198 PMID: 2348175 Document Number: 348222
Washing hair with shampoo results in an accumulation of shampoo components in the hair. Hair of individuals using different shampoos can be distinguished by analysis of shampoo residues. A method for extraction and analysis of such residues is presented. The hair is extracted using a methanol/water mixture, and the extract is analyzed by reverse-phase high-pressure liquid chromatography (HPLC). The detector system consists of two ultraviolet (UV) detectors connected in series. The method is nondestructive to hair and is sensitive enough to be applied to a single hair 5 to 10 cm in length. Residues from hair balsams are analyzed by this technique as well. The use of this method in forensic science examination of human head hair is demonstrated.