The determination of the nature of the interaction of neutral Mn2+-dependent DNAse with DNA by immunoelectron microscopy

Vinter, V.G.; Debus, N.; Abramova, Z.I.; Zotkina, N.L.; Vinter, V.G; Debus, N; Abramova, Z.I; Zotkina, N.L

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The determination of the nature of the interaction of neutral Mn2+-dependent DNAse with DNA by immunoelectron microscopy

Vinter, V.G.; Debus, N.; Abramova, Z.I.; Zotkina, N.L.

Tsitologiia 32(1): 94-96

1990

ISSN/ISBN: 0041-3771

PMID: 2165284

Document Number: 347926

The interaction of neutral DNAase with native and denatured DNA was shown by immunoelectron microscopy method with the help of colloidal gold. The neutral DNAase of the rat liver nuclear chromatin is absorbed both to denatured DNA, in which the denatured regions are arranged at 5'-3' ends, and to DNA in which these regions are distributed along the whole molecule.