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Localization of sodium-potassium adenosine triphosphatase in sheep myocardium by immunoelectron microscopy

Kossenjans, W.; Ashraf, M.

Journal of Submicroscopic Cytology and Pathology 20(1): 53-58

1988

ISSN/ISBN: 1122-9497
PMID: 2836051
Document Number: 322789
Immunohistochemical localization of sodium and potassium dependent adenosine triphosphatase (Na,K-ATPase) employing polyclonal antibodies was carried out on sheep myocardium. The tissue was fixed with glutaraldehyde or a paraformaldehyde/lysine/periodate combination fixative and embedded in different embedding media. Ultrathin sections were labeled with rabbit anti-sheep Na,K-ATPase antiserum followed by sheep anti-rabbit immunoglobulin complexed with colloidal gold. Glycol methacrylate embedded tissue provided good structural preservation and showed specific immunohistochemical labeling. Analysis of the gold particle distribution showed a significantly higher number of Na,K-ATPase immunoreactive sites associated with cell membrane and T-tubules.

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