Changes in the concentrations of hydroxyproline, glycine and serine in the plasma of haemodialysis patients undergoing erythropoietin therapy
Riedel, E.; Nündel, M.; Algermissen, B.; Hampl, H.; Scigalla, P.; Stabell, U.
Journal of Clinical Chemistry and Clinical Biochemistry 27(11): 851-856
1989
ISSN/ISBN: 0340-076X PMID: 2514250 Document Number: 347358
The concentrations of proline, hydroxyproline, glycine and serine were determined in the plasma of 39 haemodialysis patients and 18 healthy subjects, using liquid chromatography with fluorescence detection. Plasma concentrations of the N-terminal immunoreactive parathyrin were also measured. In haemodialysis patients, the plasma concentrations of glycine (p < 0.01), hydroxyproline (p < 0.05) and proline (p < 0.10) were significantly increased, whereas the serine concentrations (p < 0.01) were decreased, compared with those of the healthy controls. Haemodialysis patients showed greatly elevated plasma N-terminal immunoreactive parathyrin values (> 30 pmol/l), which showed a significant correlation with the hydroxyproline values (r = 0.79). Fourteen haemodialysis patients received erythropoietin therapy. In these patients, changes in the concentrations of plasma amino acids were observed up to one year after the beginning of therapy. In the course of the erythropoietin therapy, the plasma concentrations of glycine (p < 0.05) and hydroxyproline (p < 0.10) of the haemodialysis patients decreased, whereas the concentration of serine increased (p < 0.05) to approximately normal values. The results indicate that erythropoietin therapy leads to a normalization of amino acid metabolism.