Chemical properties of lipopolysaccharide (LPS) isolated from Vibrio anguillarum PT514
Iguchi, T.; Kondo, S.; Hisatsune, K.
Nihon Saikingaku Zasshi. Japanese Journal of Bacteriology 44(6): 805-812
1989
ISSN/ISBN: 0021-4930 PMID: 2621792 Document Number: 347074
Vibrio anguillarum, one of the causative agents of fish vibriosis, is serologically and biochemically divided into three groups (A, B and C). The chemical composition and molecular architecture of lipopolysaccharide (LPS) isolated from V. anguillarum PT 514, which belongs to serogroup B, were investigated. The LPS contianed glucose (Glc), frucrose (Fru), L-glycero-D-mannoheptose (L-D Hep), glucosamine (GlcN) and 4-amino-4,6-dideoxyglucose as sugar constituents in molar ratios of 8.9:0.7:3.0:1.1:1.6. Sephadex G-50 gel chromatography of a degraded polysaccharide fraction separated from the LPS by 5% acetic acid hydrolysis suggested that the O-specific polysaccharide region consists of, in average, as much as 29 moles of Glc per 3 moles of L-D Hep, while the core polysaccharide contains at least Glc, L-D Hep and GlcN in molar ratios of 3.2:3.0:0.2. Fru and 4-amino-4,6-dideoxyglucose components were released from LPS on weak-acid hydrolysis, indicating that PT 514 LPS is distinguishable from those of Vibrio anguillarum beloning to the other serogroups. 2-Keto-3-deoxyoctonate (KDO), a common sugar constituent of gram-negative bacterial LPS, was not detected by Weissbach's color reaction under the conventional hydrolysis condition, but O-phosphoryl KDO was found in the strong-acid hydrolysate (4 M HCl, 100 C, 45 min). This substance was identical, at lest in high-voltage paper electrophoresis, to 5-O-phosphoryl KDO. Lipid A isolated from the LPS contained C12:0, C14:0, C16:0, C16:1, C18:1, 3-OHC12:0 and 3-OHC14:0. The LPS showed endotoxic activity in limulus lysate assay to the same extent as those of V. cholerae NIH 41 (Ogawa) and 569B (Inaba).