Phosphoseryl-tRNA in Escherichia coli
Mizutani, T.; Maruyama, N.; Kurata, H.
Nucleic Acids Symposium Series 21: 25-26
1989
ISSN/ISBN: 0261-3166 PMID: 2481840 Document Number: 346325
Two seryl-tRNAs, which were prepared from natural suppressor tRNA-rich fractions on a pattern of chromatography on Sephadex A-50, were phosphorylated by a tRNA kinase in Escherichia coli B. A part of phosphate on the tRNA was confirmed to be phosphoserine. Phosphoseryl-tRNA is universal in bacteria and vertebrates. Phosphoseryl-tRNA should be an intermediate from seryl-tRNA to selenocysteyl-tRNA.
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