The effect of selected irritants on suspended keratinocytes using double vital staining
Heise, P.; Heise, H.; Wohlrab, W.
Dermatologische Monatschrift 175(10): 647-654
1989
ISSN/ISBN: 0011-9083 PMID: 2480254 Document Number: 345693
Loss of vitality of keratinocytes after influence of strong allergens and irritants was examined. Cell viability was determined by trypan blue exclusion assay and eosintyrode solution. The results of the two methods harmonize essentially, but obviously, single noxa can lead to changes of the dye that exclude the use for an appropriate test. On principle, the method is suitable for testing the substances. The toxic final concentration should constantly be ascertained. Still, the results are recommended to be guaranteed through other methods.