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Identification of specific binding sites for vasoactive intestinal peptide in rat testis Leydig cells and study of developmental changes

Hueso, C.; Carmena, M.J.; Prieto, J.C.

Biochemistry International 19(5): 951-958

1989

ISSN/ISBN: 0158-5231
PMID: 2635855
Document Number: 344701
The interaction of vasoactive intestinal peptide (VIP) with isolated Leydig cells from rat testis was time- and temperature-dependent, as well as saturable and specific. Scatchard analysis suggested the presence of both high- and low-affinity binding sites with KD values of 1.7 and 43 nM, respectively, and receptor concentrations of 35 and 1394 fmol VIP bound/mg protein in mature (3- to 6-month old) rats. When considering pubertal (45-day old) rats, the affinities were similar but the binding capacities showed considerably lower values (25 and 193 fmol VIP bound/mg protein) indicating that VIP receptors are subject to developmental changes during animal maturation.

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