Determination of the enzyme activity in tissue homogenates and biological liquids
Brestkin, A.P.; Dmitrienko, O.V.; Kostrova, V.M.; Kuznetsova, P.L.; Nikol'skaia, E.B.
Laboratornoe Delo 4: 13-15
1989
ISSN/ISBN: 0023-6748 PMID: 2470954 Document Number: 343923
The described method for measuring the enzymic activities in tissue homogenates and biological fluids is based on the separation of the space with the biological fluid, where the enzymic reaction takes place, from the space with the substrate solution, where the analytical effect is measured, by a semipermeable membrane. The authors present the results of measurements of monoamine oxidase activity in rat liver mitochondria homogenate, of acetylcholinesterase activity in mouse brain homogenate, and of cholinesterase activity in cow blood serum.