Isolated symplasts and stromal syncytial buds of the normal human placenta at 6 to 14 weeks of pregnancy
Voloshchuk, I.N.; Fidler, R.; Milovanov, A.P.; Verbitskiĭ, M.S.; Zakharova, O.I.
Arkhiv Anatomii Gistologii i Embriologii 97(11): 70-76
1989
ISSN/ISBN: 0004-1947 PMID: 2483609 Document Number: 343824
Formation of isolated symplasts (IS) ("deported trophoblast", according to English nomenclature) and stromal syncytial buds (SSB) has been studied in 10 normal patients at pregnancy of 6-14 weeks by means of the indirect immunoperoxidase method. Monoclonal antibodies against beta-subunit of the human chorionic gonadotropin (beta-HCG), serial paraffin slices, stained with hematoxylin-eosin, semithin slices, stained with toluidin blue-pyronine and scanning electron microscopy have been used. A great amount of syncytiotrophoblastic processes and IS, analogous by their structure to syncytiotrophoblast are revealed. Presence of beta-HCG in the syncytiotrophoblast and its absence in the cytotrophoblast are proved. Concentration of beta-HCG in the IS is higher than in the syncytiotrophoblast of the villi. Maximal concentration of beta-HCG in the SSB is described for the first time. The functional role of the IS and SSB is discussed in the system mother-placenta-fetus.