Altered calcium homeostasis in carbon tetrachloride exposed rat hepatocytes
Andrabi, K.; Kaul, N.; Ganguly, N.K.; Dilawari, J.B.
Biochemistry International 18(6): 1287-1295
1989
ISSN/ISBN: 0158-5231 PMID: 2751689 Document Number: 342019
In situ perfusion of rat liver with carbon tetrachloride (10-15 mM at a final concentration of lul/ml of mineral oil) resulted in a significant (p less than 0.01) loss of microsomal Ca2+ over 5-15 min of exposure. Mitochondrial Ca2+ content increased sharply (2 fold) with concomitant increase in the cytosolic free Ca2+. The changes were associated with a significant (p less than 0.01) decrease in plasma membrane Ca2+ - ATPase activity, reflected by impaired Ca2+ efflux and Ca2+ exchange properties. Our data indicates that impairment of plasma membrane Ca2+ flux contributes substantially to the overall disruption of calcium homeostasis induced by carbon tetrachloride.