NAD participation in protecting DNA from damaging factors
Muliavko, N.A.; Petik, A.V.; Donchenko, G.V.
Biokhimiia 54(1): 140-144
1989
ISSN/ISBN: 0320-9725 PMID: 2524217 Document Number: 338528
It was found that autolytic degradation of DNA during the isolation of nuclei under conditions of an in situ stimulated NAD biosynthesis is decreased to 2.5% in comparison with 5.2% in control. The increase in the NAD-pyrophosphorylase activity and the intranuclear content of NAD was almost 3-fold, whereas the amount of the acid-soluble DNA material was decreased 2-fold. The degree of DNA damage was increased up to 23% after induction with ethidium bromide. Under these conditions a preliminary in vivo stimulation of NAD biosynthesis by nicotinamide diminished the amount of the acid-soluble material. The levels of intranuclear NAD as well as the NAD-pyrophosphorylase and poly-(ADP-riboso)polymerase activities were essentially affected.