Ca2+-phospholipid-dependent phosphorylation of cytosol and membrane proteins of the brain in light craniocerebral trauma
Kucherenko, S.N.; Ostapchenko, L.I.; Vasil'ev, A.N.
Zhurnal Nevropatologii i Psikhiatrii Imeni S.S. Korsakova 89(12): 11-14
1989
ISSN/ISBN: 0044-4588 PMID: 2633560 Document Number: 338277
Immediately after light craniocerebral trauma (LCCT) the system of Ca2+, phospholipid-dependent phosphorylation of cytosol and microsomes of diencephalic-brainstem regions was impaired. The system of regulation of activity of C-kinase by phosphatidylserine was upset in the course of posttraumatic period and was different for cytosol and hemispheres. The increase in phosphorylation upon addition of exogenic C-kinase to incubation medium was evident only with stimulation of endogenic phosphorylation by phosphatidylserine. The decrease in Ca2+, phospholipid-dependent phosphorylation was characteristic of all fractions studied 1 day after trauma. After 14 days, the enzyme activity did not return to baseline. The authors suggest that C-kinase participates in trigger mechanisms of posttraumatic disease.