A study of sex identification of trace, dried bloodstains using a Y-chromosome-specific deoxyribonucleic acid (DNA) probe

He, Z.N.; Jiang, X.H.; Lu, S.H.; Wang, G.L.; Zhu, Y.W.; Wang, S.W.; Shen, Y.; Gao, Q.S.; Liu, J.Z.; Wu, G.Y.; He, Z.N; Jiang, X.H; Lu, S.H; Wang, G.L; Zhu, Y.W; Wang, S.W; Shen, Y; Gao, Q.S; Liu, J.Z; Wu, G.Y

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A study of sex identification of trace, dried bloodstains using a Y-chromosome-specific deoxyribonucleic acid (DNA) probe

He, Z.N.; Jiang, X.H.; Lu, S.H.; Wang, G.L.; Zhu, Y.W.; Wang, S.W.; Shen, Y.; Gao, Q.S.; Liu, J.Z.; Wu, G.Y.

Journal of Forensic Sciences 34(2): 346-351

1989

ISSN/ISBN: 0022-1198

PMID: 2708955

Document Number: 338052

A new method is discussed which examines trace, dried bloodstains by gel in situ hybridization using a Y-chromosome-specific deoxyribonucleic acid (DNA) probe to determine the sex of the bloodstain for forensic medicine application. The complete DNA is transferred directly by electrophoresis onto the gel intact, bypassing the possibilities of impurities contaminating the sample and of DNA degradation. The method has proven accurate for small (2.5-mm-diameter) samples aged up to eight years and is quick, simple, and easily read.