Stimulation of multiplication of chick embryo cultured cells after an increase in density of the cellular population
Gasparian, G.G.; Grigorian, R.M.
Tsitologiia 31(4): 440-446
1989
ISSN/ISBN: 0041-3771 PMID: 2756570 Document Number: 336987
The secondary culture of chick embryo cells in 2-3 days after seeding was super-inoculated with homologous cells. Suspended cells adhere and spread on the cell layer whereby the culture density increases quickly. After adhesion of exogenous cells to the layer the stimulation of cell proliferation takes place. This activation is not connected with methodical manipulations or with the influence of conditioned medium factors. The results suggest that the increase in cell number itself does not arrest cell multiplication. It is proposed that the known phenomenon of blocking cell proliferation in dense cultures cannot be attributed only to effects of high cell density.