Diagnosis of congenital CMV infection by specific CMV IgM antibody in cord serum with ELISA
Guo, T.L.; Tang, Z.Y.; Wu, Y.X.; Yao, Y.J.; Li, W.R.
Hua Xi Yi Ke da Xue Xue Bao 20(4): 422-425
1989
ISSN/ISBN: 0257-7712 PMID: 2560989 Document Number: 336800
Cytomegalovirus IgM (CMV IgM) antibodies of 582 cord sera from 6 hospitals in Chengdu were detected by direct ELISA and indirect ELISA. Twenty-seven cases with CMV IgM antibody positive were detected from 582 cord sera. Twenty-five and 15 cases were found to have CMV IgM antibody by direct ELISA and indirect ELISA, respectively (P greater than 0.05). Indirect ELISA for detection CMV IgM antibody was only interfered by high concentration of CMV IgG and rheumatoid factor. Children with CMV IgM antibody positive were followed-up to 5-6 months. Hearing loss was detected by impedance audiology in only 2 cases. But the other physical and mental examinations were normal. The result showed that the prevalence of congenital CMV infection is 4.6% in our study and direct ELISA method is more specific and sensitive than indirect ELISA method to detect CMV IgM antibody in cord serum.