Structural analysis of carbohydrate chains of normal and myeloma immunoglobulins G using HPLC
Arbatskiĭ, N.P.; Martynova, M.D.; Aleshkin, V.A.; Derevitskaia, V.A.
Bioorganicheskaia Khimiia 15(2): 175-180
1989
ISSN/ISBN: 0132-3423 PMID: 2742610 Document Number: 335613
A comparative analysis of carbohydrate chains from human normal IgG and myeloma IgG (subclass 4) was carried out using HPLC. It is shown that both IgGs contain the same carbohydrate chains (biantennary and bisected) but the relative amount of bisected and incomplete chains (with fewer terminal galactose residues) is higher in myeloma IgG4. Two earlier unknown minor oligosaccharides were isolated from normal IgG and structurally studied by means of the partial hydrolysis and the Smith degradation.