Acyclovir susceptibility of herpes simplex virus isolates at King Chulalongkorn Memorial Hospital, Bangkok
Sangdara, A.; Bhattarakosol, P.
Journal of the Medical Association of Thailand 91(6): 908-912
2008
ISSN/ISBN: 0125-2208 PMID: 18697392 Document Number: 3354
Objective: To determine the ACV susceptibility in Thai HSV clinical isolates.Material and Method. One hundred thirty HSV isolates from the Virology Laboratory Unit, King Chulalongkorn Memorial Hospital, Bangkok, Thailand had typing done by immunofluoresent assay using monoclonal antibody specific to either HSV-1 or HSV-2, Their sensitivity to ACV (IC50) was determined by plaque reduction assay.Results: The IC50 of 77 HSV-1 isolates ranged from 0.07-0.97 mu g/ml and that of 53 HSV-2 isolates was 0.13-1.66 mu g/ml. The standard HSV-1 (KOS) and HSV-2 (Baylor 186) were included in each run. The mean +/- standard deviation (SD) of ACV IC50 among HSV-1 and HSV-2 isolates were 0.38 +/- 0.23 and 0.50 +/- 0.32 mu g/ml while that of standard HSV-1 and HSV-2 were 0.45 +/- 0.13 and 0.57 +/- 0.04 mu g/ml. Statistically significant difference between IC50 of HSV-1 and HSV-2 isolates was indicated (p = 0.02).Conclusion: No ACPI HSV has been detected and ACV susceptibility of HSV-2 has more resistance than that Of HSV-1.
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