Differential secretion of stimulatory-inhibitory CFU-GM activity by stromal cells from different compartments of mouse bone femur
Gupta, V.
Experimental Hematology 17(2): 188-190
1989
ISSN/ISBN: 0301-472X PMID: 2463932 Document Number: 335241
We have recently reported the beneficial effect of low oxygen concentration on the growth of fibroblast colony-forming units (CFU-F) isolated from the shaft of the femur (fraction 1), the endosteum (fraction 2), and the compact bone (fraction 3). We now report that the conditioned medium obtained from the CFU-F in the same experiments exhibited stimulatory activity from the compact bone while expressing inhibitory activity from the femoral shaft and endosteum for granulocyte-macrophage CFU (CFU-GM). These data support the presence of a spatial regulatory role for fibroblast-like cells within compartments of mouse bone for CFU-GM.