Monoclonal antibodies to Legionella pneumophila cytolysin
Spitsyn, S.V.; Barkhatova OI, A.L.; Drobyshevskaia, E.I.; Belyĭ, I.F.; Tartakovskiĭ, I.S.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1989(10): 83-87
1989
ISSN/ISBN: 0372-9311 PMID: 2692353 Document Number: 335191
The fusion of spleen cells, taken from BALB/c mice immunized with the purified preparation of Legionella pneumophila cytolysin, with cells Sp2/0 and NP has been carried out. As a result, hybridoma cells producing IgG1, IgG3 and IgM antibodies to this protein have been obtained. All monoclonal antibodies (McAb) thus obtained react with L. pneumonphila strain lysates in the precipitation test, while IgG3 and IgM antibodies react with erythrocyte diagnosticums prepared from the lysate of L. pneumophila cells in the hemagglutination test. In the Western blot assay, McAb react with the 37 KD protein (cytolysin) and a number of other proteins from L. pneumophila cultures and L. pneumonphila cells lysate, but do not react with the species-specific protein with a molecular weight of 29 KD contained in the outer membrane of L. bozemany, as well as with other species: L. bozemany, L. dumoffii, L. longbeachae, L. micdadei. The possibility of using these McAb conjugated with FITC and peroxidase for the rapid diagnosis of Legionella infection is shown.