Cytolysin as an immunoserological marker of Legionella pneumophila
Tartakovskiĭ, I.S.; Belyĭ, I.F.; Vertiev, I.V.; Nagaev, I.G.; Prozorovskiĭ, S.V.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 5: 58-61
1988
ISSN/ISBN: 0372-9311 PMID: 2458000 Document Number: 324680
Coagglutination test (CoaT), radioimmunoassay (RIA) and ELISA were used for detection of cytolysin produced by L. pneumophila. The sensitivity of RIA was 100 ng/ml, CoaT--10-20 ng/ml, ELISA--1 ng/ml. To determine cytolysin production among various strains and species of Legionella, the authors studied bacterial ultrasonic lysates. All 11 strains of L. pneumophila tested were cytolysin-positive. L. longbeachae, L. bozemanii and L. dumoffii strains failed to produce cytolysin. The authors believe that an antigen of cytolysin can be used for identification of L. pneumophila.