Chronic infection of cultured cells by rabies viruses
Cokić-Damjanović, J.
Medicinski Pregled 42(1-2): 45-47
1989
ISSN/ISBN: 0025-8105 PMID: 2761478 Document Number: 334819
The culture of the continuous cellular line BHK 21 C13 was infected in the routine way by rabies virus, a kind of fixed CVS virus. Through 100 passages, the virus was (parallely) isolated and titrated in white mice and in the cellular culture by the technique of fluorescent TFA-a antibodies. In respect to mice, infectiveness of cells was less marked, while through further passages infectiveness of cells increased, while the titre in mice decreased. In the course of 100 passages, a repeat infection of cellular culture was not performed at all, but the virus multiplied together with the chronically infected cells.