Photogeneration of singlet molecular oxygen by the components of hematoporphyrin IX derivative
Egorov, S.Iu.; Tauber, A.Iu.; Krasnovskiĭ, A.A.; Nizhnik, A.N.; Nokel', A.Iu.; Mironov, A.F.
Biulleten' Eksperimental'noi Biologii i Meditsiny 108(10): 440-442
1989
ISSN/ISBN: 0365-9615 PMID: 2532043 Document Number: 331016
The photosensitized luminescence of single molecular oxygen has been studied in aqueous and alcoholic solutions of hematoporphyrin IX (HP) and di- and oligomeric components of "hematoporphyrin derivative" (photofrin II) which is nown to be used as a drug in photodynamic tumor therapy. The quantum yields of 1O2 generation of (.gamma.DELTA.) by these compounds have been determined. It was found that the highest .gamma.DELTA. values are characteristic of alcoholic and micellar detergent aqueous solutions. In detergent-free aqueous solutions contianing mainly associated porphyrin molecules, .gamma.DELTA. is much lower (5-30%), polymeric photofrin components being considerably less active than HP. Both localization of porphyrins in hydrophobic locuses and high photosensitizing activity in lipid phase are supposed to play the key role in tumor photodestruction.