The relation of changes in the surface markers and the induction of thymocyte functional activity under the action of humoral thymic factors
Iarilin, A.A.; Nikonova, M.F.; Lutsik, M.D.
Tsitologiia 31(8): 945-954
1989
ISSN/ISBN: 0041-3771 PMID: 2573967 Document Number: 330266
Mouse thymocytes were fractionated on PNA+- and PNA-cells. The PNA+-fraction was treated by tactivin--a preparation of thymic hormones. The treatment resulted in the loss of PNA-receptors from the surface of 10-12% of PNA+-cells. The treated suspension was again fractionated by the same method. PNA-thymocytes derived from both the first and the second procedure of fractionation expressed less Thy-1.2 antigen and SBA-receptors than PNA+-cells. Primary and induced PNA-thymocytes were able to produce IL-2 after mitogen stimulation. The data can be interpreted as an evidence of the ability of thymic hormones to induce not only a phenotypical but also a functional maturation of PNA+-thymocytes and of linkage of all the observed signs of maturation.