Distribution and biotransformation of labelled pantothenate in cytoplasmic and mitochondrial fractions of the rat liver with a deficiency of pantothenic acid
Reziapkin, V.I.; Gurinovich, V.A.; Chiger, V.B.; Moiseenok, A.G.
Voprosy Pitaniia 5: 50-53
1989
ISSN/ISBN: 0042-8833 PMID: 2609557 Document Number: 329723
Experiments were conducted on white rats given synthetic rations devoid of pantothenic acid during 10 weeks. Intensification of 14C-pantothenate deposition was recorded 30 min and 4 h after its intraperitoneal administration. The mitochondrial fraction of the liver accumulated the isotope in time. High-performance liquid chromatography used for separation of the vitamin labeled metabolites has revealed phosphopantothenate (pantothenate), phosphopantothein, CoA and dephospho-CoA (pantetein) in the liver homogenate, while in the mitochondria extracts only CoA and dephospho-CoA (pantetein) were detected. It has been suggested that dephosphorylation of pantothenate metabolites and rapid transformation of phosphopantetein into CoA may take place during the separation of the fractions.