Isolation of nucleotide sequences, possessing enhancer functions from the Saccharomyces cerevisiae genetic library
Polishchuk, A.G.; Nevzgliadova, O.V.; Tereshin, I.M.; Soĭdla, T.R.
Molekuliarnaia Biologiia 23(1): 279-285
1989
ISSN/ISBN: 0026-8984 PMID: 2544796 Document Number: 328186
We propose a method for isolation of enhancer-like sequences from a yeast genomic library. The method was used to identify DNA inserts capable of increasing bacterial bla gene expression when located downstream from the transcription initiation site. Plasmids carrying different fragments of one such insert were used to localize the enhancer-like function on a 1.2 kb fragment.