A method for obtaining a long-lived intestinal tissue culture
Kuberger, M.B.; Sviridov, D.D.; Izachik, N.A.; Nazhimov, V.P.; Izachik, I.A.
Biulleten' Eksperimental'noi Biologii i Meditsiny 107(4): 503-505
1989
ISSN/ISBN: 0365-9615 PMID: 2720172 Document Number: 327795
The interest in the studies of the intestines using the method of tissue organ culture has considerably grown in recent years. It can be explained by the great possibilities of obtaining unique data about the state of intestines in normal and pathological condition, e.g. malabsorption syndrome. The paper deals with the method modified by the authors to obtain long-living (24 hours) intestinal tissue organ culture. The investigations used bioptic sections which were obtained by jejunoscopy with spot biopsy of children suffering from intestinal malabsorption. The viability of the explants was proved by histological and histochemical tests. The promise held by the methods is emphasized.