Effect of chloramphenicol on DNA repair synthesis in fetal liver cells of rats
Wang, A.P.; Wang, X.P.
Zhongguo Yao Li Xue Bao 10(6): 543-545
1989
ISSN/ISBN: 0253-9756 PMID: 2641854 Document Number: 326918
The inhibitory effect of chloramphenicol (CAP) on the DNA repair synthesis were studied on a primary cell culture of fetal liver tissue of rats. The fetuses were taken out with the umbilical cords and the placenta: then, the fetal livers were injected with a balanced salt solution and Type I collagenase through the umbilical vein. At the same time, the blood of the fetal livers were drained through the umbilical artery. The cells were detached at 10.degree. C with a steel mesh. Chloramphenicol 1.6-403.8 mmol/L did not show significant increase in DNA repair synthesis. The unscheduled DNA synthesis (UDS) was active when the positive control 7, 12-dimethylbenzanthrancene (DMBA) was added alone to the culture in autoradiographic assay, but it was inactive when DMBA was added together with CAP. Therefore, it may be assumed that CAP has an inhibitory effect on the DNA repair synthesis.