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Transposition of insertional sequence IS21 in E. coli cells activated by an exogenous promotor

Dubeĭkovskiĭ, A.N.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1: 19-24

1989

ISSN/ISBN: 0208-0613
PMID: 2543920
Document Number: 326792
Transpositional activity of the IS21-element was found to be activated during its cloning in the pUC18 vector plasmid. The activated IS-element restores ability to transposition of the deficient IS21-element present in the same cell. Simultaneously with the activated transposition the increase in frequencies of precise exclusion of IS-element is registered. The activation of IS-element is concluded to be directed by exogenous promoter.

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