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The role of binding proteins in radioimmunologic determination of the free thyroxine concentration using thyroxine analog methods: thyroxine immunoextraction versus thyroxine analog immunoextraction

Passath, A.

Wiener Medizinische Wochenschrift 139(5): 98-102

1989

ISSN/ISBN: 0043-5341
PMID: 2471363
Document Number: 326625
Amerlex FT4-analogue one step RIA was investigated to estimate the 125-Iodine thyroxine immunoextraction in sera with normal and abnormal concentrations of binding proteins. The following results were found: Amerlex analogue FT4 RIA equals an equilibrium-immunoassay. Thyroxin-analogue tracer is strongly bound to an albumin (80%). During the assay procedure, there is no analogue binding to serum proteins. If serum and FT4-antibody diluted in an adequate manner, FT4-analogue values in all serum dilution steps are determined identically. FT4-analogue values are influenced only by thyroxine, bound to albumin and/or prealbumin and not by TBG-bound thyroxine. Serum albumin concentration does not influence FT4-analogue values.

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