Role of the LFA3-CD2 interaction in human specific B cell differentiation
Emilie, D.; Wallon, C.; Galanaud, P.; Fischer, A.; Olive, D.; Delfraissy, J.F.
Journal of Immunology 141(6): 1912-1918
1988
ISSN/ISBN: 0022-1767 PMID: 2459195 Document Number: 325815
We examined the role of the lymphocyte function-associated (LFA)3 molecule in human B cell response. A mAb to this molecule did not influence B cell proliferation induced by anti-mu antibody and IL. In contrast, the same mAb inhibited the specific T-dependent B cell response induced by a particulate Ag. In the same line, two anti-CD2 mAb (directed toward the T11-1 and T11-2 epitopes) inhibited this response, whether used alone or in association. These inhibitions took place at an early stage of the response, and anti-LFA3 and anti-CD2 mAb acted on B cells and T cells, respectively. In contrast, when T cell help was provided by exogenous IL-2, the B cell response was resistant to the inhibitory effect of anti-LFA3 mAb. Taken together, these results indicate that the LFA3-CD2 pair play a major role in the direct T-B interaction required for T cell help.