Stimulation of phospholipase C activity by 4-hydroxynonenal; influence of GTP and calcium concentration
Rossi, M.A.; Garramone, A.; Dianzani, M.U.
International Journal of Tissue Reactions 10(5): 321-325
1988
ISSN/ISBN: 0250-0868 PMID: 3250944 Document Number: 325592
4-Hydroxynonenal (HNE), a major aldehyde end-product of lipid peroxidation, induces in vitro a rapid stimulation of rat liver PIP2-phospholipase C. At physiological Ca2+ concentration the effect of the aldehyde is strongly potentiated by guanosine thiotriphosphate (GTP gamma S); GPT gamma S; at higher Ca2+ levels the acceleration of PIP2 breakdown induced by the aldehyde reaches very high values, but is no longer modulated by the presence of GTP gamma S. As the concentration of the aldehyde used (1 micromolar) can be actually reached in tissues, the effects shown in vitro are likely to occur in vivo.