Preservation of the circadian rhythm of human lymphocytes under in vitro conditions
Gamaleia, N.F.; Shishko, E.D.; Chernyĭ, A.P.
Biulleten' Eksperimental'noi Biologii i Meditsiny 106(11): 598-600
1988
ISSN/ISBN: 0365-9615 PMID: 3196863 Document Number: 325479
The proportions of E-rosette-forming cells on the 9th, 13th, 17th, 21st, 1st and 5th h were determined in the blood of healthy donors divided into 3 groups (4 men in each). I--the blood was taken at every specified time point and the rosette formation was tested immediately; II--the blood was taken at hour 9, stored at +20 degrees C throughout the experiment and used for the tests at the same time points; III--the blood taken at hour 9, the mononuclear cells were obtained and kept throughout the experiment for the determination of rosette formation at the same time points. In all the three groups similar circadian curves were obtained with a peak at 13th and a minimum at the 1st hour. It is believed that lymphocytes possess oscillator(s) of the activity of receptors that are used for cell subset identification.