Intravitreal injection of human anti-pseudomonas globulin in pseudomonas endophthalmitis in rabbits
Hatano, H.; Tanaka, N.; Kawano, M.; Matsumoto, K.; Isobe, Y.; Inoue, K.
Nippon Ganka Gakkai Zasshi 92(12): 2120-2128
1988
ISSN/ISBN: 0029-0203 PMID: 3266443 Document Number: 325374
The effect of the intravitreal injection of anti-pseudomonas globulin in pseudomonas globulin in pseudomonas endophthalmitis was investigated using albino rabbits. In the first experiment we injected a mixture of five viable cells of Pseudomonas aeruginosa and 10 mg of human anti-pseudomonas globulin into the vitreous cavities of the right eyes of six rabbits. The left eyes were injected with pseudomonas and human albumin as the control. They were clinically followed for five days and then enucleated for vitreous culture and histology. The right eyes showed no clinical findings except one eye, whereas all left eyes progressed to endophthalmitis. Pseudomonas were recovered from one right eye and all left eyes. The left eyes showed typical histopathological findings of panophthalmitis with marked intraocular inflammation and destruction, which were not seen in the right eyes, except for one eye. In the second experiment the vitreous cavities of six rabbits were injected with five cells of pseudomonas, and one hour later the right and left vitreous cavities were injected with 10 mg of globulin and albumin respectively. They were clinically followed for six days and ERGs were recorded. The viable cell numbers of pseudomonas in the vitreous cavities were also counted at given times using another nine rabbits injected in the same way as above. All the eyes developed endophthalmitis, but the right eyes showed milder inflammation than the left eyes. ERGs of all left eyes became extinguished at 48 hours but those of the right eyes did not. The viable cell numbers of the right eyes were much smaller than those of left eyes at all given times. The above indicates that the intravitreal injection of anti-pseudomonas globulin significantly suppressed pseudomonas endophtalmitis in rabbits.