Suppressive action of the influenza virus on the bone marrow stem cells of mice
Semenkov, V.F.; Lavrov, V.F.; Khodakova, L.P.
Voprosy Virusologii 33(4): 416-419
1988
ISSN/ISBN: 0507-4088 PMID: 2904194 Document Number: 325098
The effect of pathogenic and noninfectious influenza viruses with different surface antigens on colony-forming stem cells of (CBA X C57BL/6)F1 mice was studied. Bone marrow cells were infected with A/PR8/34 (H1N1)/A/Krasnodar/101/59 (H2N2) viruses, a recombinant A/PK-6-3 (H2N1) strain thereof, A/Khabarovsk/933/77 (H1N1) strain; then they were inoculated intravenously to irradiated (820 rad) syngeneic recipients, and colony-forming units (CFU) were determined in the spleens 9 days after injection. Inoculation of bone marrow cells (2 X 10(5) per mouse) to the irradiated recipients immediately after mixing with a live A/Krasnodar virus heated at 56 degrees C, live A/PK-6-3, and A/Khabarovsk viruses resulted in suppression of formation of CFU and marked atrophy of the recipients' spleens. There was no suppression after the use in these experiments of the pathogenic A/PR/8/34 strain. Inhibition of CFU production in the spleen was observed after inoculation of the recipients with bone marrow cells incubated for 1 hour at 37 degrees C with A/PR/8/34, A/Krasnodar/101/59 viruses and the recombinant strain. Possible mechanisms of the suppressive effect of influenza A virus on CFU production in mice are discussed.