Ultrastructural characterization of organ cultures of adrenal glands of newborn pigs
Tron'ko, N.D.; Bogdanova, T.I.; Turchin, I.S.
Problemy Endokrinologii 34(5): 65-69
1988
ISSN/ISBN: 0375-9660 PMID: 3217394 Document Number: 322323
Electron microscopic investigation of newborn pig adrenal organ cultures performed for determination of optimum time for xenotransplantation, has shown that the most viable and functional adrenocorticocytes preserving signs of zonal specificity are revealed on the 5th day of cultivation. Later on (the 15th-30th day) morphological changes indicate increasing dedifferentiation of most secretory cells, the capacity to corticosteroidogenesis (judging by the state of steroid-synthesizing organelles) in some adrenocorticocytes being preserved up to 25-30 days. Thus, the most suitable time for xenotransplantation of adrenal organ cultures is the 5th day.