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Interaction of rat testis protein, TP, with nucleosome core particle

Singh, J.; Rao, M.R.

Biochemistry International 17(4): 701-710

1988

ISSN/ISBN: 0158-5231
PMID: 3240317
Document Number: 321192
Circular dichroism studies have revealed that addition of testis specific protein, TP in vitro to rat testes nucleosome core particle resulted in a decrease in the compaction of the core particle DNA. This was also corroborated by thermal denaturation analysis. Addition of TP to nucleosome core particle resulted in the conversion of a biphasic transition towards a single phase. However, at the same time there was a 20% reduction in the overall hyperchromicity of core particle DNA at core particle to TP molar ratios of 1:2 and 1:3. These observations along with our earlier report, showing the DNA melting properties of TP, suggest that TP may play an important role in the disassembly process of nucleosome core particle during spermiogenesis.

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Carless, J.E.; Ocran, J. 1972: Relations between particle shape, particle interaction and some rheological properties of hectorite dispersions Journal of Pharmacy and Pharmacology 24(8): 637-644
Marcum, J.A.; Thompson, M.A. 1991: The amino-terminal region of a proteochondroitin core protein, secreted by aortic smooth muscle cells, shares sequence homology with the pre-propeptide region of the biglycan core protein from human bone Biochemical and Biophysical Research Communications 175(2): 706-712
Hwang, L.H.; Lin, Y.J.; Lai, W.C.; Lo, M.S. 1991: Protease-like sequence in hepatitis B virus core antigen is not involved in the cleavage processes of core protein in Escherichia coli Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 24(1): 71-83
Karpenko, L.I.; Ivanisenko, V.A.; Pika, I.S.; Chikaev, N.A.; Eroshkin, A.M.; Melamed, N.V.; Veremeĭko, T.A.; Il'ichev, A.A. 2000: Analysis of foreign epitopes inserted in HBcAG. Possible routes for solving the problem of chimeric core particle self assembly Molekuliarnaia Biologiia 34(2): 223-229
Gutiérrez-Adrianzén, O.Augusto.; Koutouzov, S.; Mota, R.Maria.Salani.; das Chagas Medeiros, M.Maria.; Bach, J-François.; de Holanda Campos, H. 2006: Diagnostic value of anti-nucleosome antibodies in the assessment of disease activity of systemic lupus erythematosus: a prospective study comparing anti-nucleosome with anti-dsDNA antibodies Journal of Rheumatology 33(8): 1538-1544
Songsivilai, S.; Dharakul, T.; Kunkitti, R.; Thepthai, C. 1996: Molecular cloning and expression of hepatitis C virus core protein and production of monoclonal antibodies to the recombinant protein Asian Pacific Journal of Allergy and Immunology 14(1): 31-41
Douglas, S.D. 1978: Alterations in intramembrane particle distribution during interaction of erythrocyte-bound ligands with immunoprotein receptors Journal of Immunology 120(1): 151-157
Khadake, J.R.; Markose, E.R.; Rao, M.R. 1994: Testis-specific histone (H1t) is not phosphorylated and has a weak interaction with chromatin Indian Journal of Biochemistry and Biophysics 31(4): 335-338
James, A.M.; Goddard, G.H. 1971: A study of barium sulphate preparations used as x-ray opaque media. I. Particle size and particle charge Pharmaceutica Acta Helvetiae 46(12): 708-720
Yasui, N. 2017: Functional analyses of specific protein-protein interaction networks using affinity clamping technology Seikagaku. Journal of Japanese Biochemical Society 89(2): 293-297
Chatton, B.; Bahr, A.; Acker, J.; Kedinger, C. 1995: Eukaryotic GST fusion vector for the study of protein-protein associations in vivo: application to interaction of ATFa with Jun and Fos Biotechniques 18(1): 142-145
Schade, M.; Oschkinat, H. 2005: NMR fragment screening: tackling protein-protein interaction targets Current Opinion in Drug Discovery and Development 8(3): 365-373
Sato, K.; Hosokawa, K. 1983: DNA-binding domain in adenovirus core protein VIi Biochemistry International 7(4): 443-448
Lai, J.; He, X.; Wang, F.; Tan, J.-M.; Wang, J.-X.; Xing, S.-M.; Shen, L.-B.; Fang, L.-Q.; Yang, P.; Tan, J.-M. 2013: Gene expression signature analysis and protein-protein interaction network construction of spinal cord injury European Review for Medical and Pharmacological Sciences 17(21): 2941-2948
Williams, R.T.; Carbonaro-Hall, D.A.; Hall, F.L. 1992: Co-purification of p34cdc2/p58cyclin A proline-directed protein kinase and the retinoblastoma tumor susceptibility gene product: interaction of an oncogenic serine/threonine protein kinase with a tumor-suppressor protein Oncogene 7(3): 423-432
Mei, G.-y.; Li, Y.; Wang, G.-r.; Zhang, B.-y.; Tian, C.; Chen, C.; Zhou, R.-m.; Wang, X.; Li, X.-l.; Wang, K.-x.; Han, J.; Dong, X.-p. 2009: Molecular interaction between PrP protein and the signal protein 14-3-3 beta Bing du Xue Bao 25(3): 208-212
Yu, L.J.; Ma, R.D.; Jiang, S.B. 1994: Effects of tubeimoside-1 on HIV core protein p24 and cytopathogenesis in vitro Zhongguo Yao Li Xue Bao 15(2): 103-106
Nicolet, C.M.; Burkholder, J.K.; Gan, J.; Culp, J.; Kashmiri, S.V.; Schlom, J.; Yang, N.S.; Sondel, P.M. 1995: Expression of a tumor-reactive antibody-interleukin 2 fusion protein after in vivo particle-mediated gene delivery Cancer Gene Therapy 2(3): 161-170
Vega, J.A.; Zubizarreta, J.J.; del Valle, M.E.; Hernández, L.C.; Bengoechea, M.E.; Pérez-Casas, A. 1990: Immunohistochemical study of cat Pacinian corpuscles: co-localization of vimentin- and S-100 protein-like in the inner core Cellular and Molecular Biology 36(4): 415-420
Sabet, S.F.; Al-Sherbiny, M.M.; Hagan, P. 2009: Identification, cloning and expression of core protein gene of hepatitis C genotype 4a Journal of the Egyptian Society of Parasitology 39(3): 769-787