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Synthesis of visual rhodopsin in a cell-free translation system. I. Influence of the structure of the synthetic bovine visual opsin mRNA on its translational efficiency

Zozulia, S.A.; Gurevich, V.V.; Shmukler, B.E.; Namochin, M.Iu.; Zviaga, T.A.

Bioorganicheskaia Khimiia 14(12): 1663-1670

1988

ISSN/ISBN: 0132-3423
PMID: 2978006
Document Number: 318374
Influence of structural changes in nontranslated regions and translation initiation site of the in vitro synthesized bovine opsin mRNA on its translational efficiency in the wheat germ cell-free system has been studied. It is shown that level of the opsin synthesis up to 30 micrograms per 1 ml of translational mixture can be attained by optimizing structure of 5'-nontranslated region.

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