Regulation of Fc epsilon receptor expression on a human monoblast cell line U937
Mayumi, M.; Kawabe, T.; Kim, K.M.; Heike, T.; Katamura, K.; Yodoi, J.; Mikawa, H.
Clinical and Experimental Immunology 71(1): 202-206
1988
ISSN/ISBN: 0009-9104 PMID: 2964957 Document Number: 317952
Fc.epsilon. receptor (Fc.epsilon. R) expression on several human cell lines (U937, RPMI 8866, HL 60, THP-1, and Molt 4) and its regulation were examined by immunofluorescent analysis using a monoclonal antihuman Fc.epsilon.R antibody, H107. Phorbol ester (PMA), recombinant gamma interferon (IFN-.gamma.) and H107 itself enhanced Fc.epsilon.R expression on a FC.epsilon.R positive cell line U937, whereas these reagents did not induce FC.epsilon.R expression on the Fc.epsilon.R negative cell lines, Molt 4, HL 60 and THP-1. Dexamethasone not only suppressed by 50% the spontaneous Fc.epsilon.R expression on U937 cells but also completely inhibited the enhancement of their Fc.epsilon.R expression on U937 cells induced by PMA, IFN-.gamma. or H107. Dexamethasone caused a little suppression of Fc.epsilon.R expression by RPMI 8866 cells. The results showed that Fc.epsilon.R expression on a human monoblast cell line U937 was up- or down-regulated by a variety of physiological or pharmacological agents. These experimental systems provide a good model for the investigation of the regulatory mechanisms of Fc.epsilon.R expression.